Secretion of Pore-Forming Peptide Toxin, Tolaasin, by Ptα Type Strains of Pseudomonas Tolaasii, but not by Ptβ Type Strains

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Purification of a pore-forming peptide toxin, tolaasin, produced by Pseudomonas tolaasii 6264.

Tolaasin, a pore-forming peptide toxin, is produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. P. tolaasii 6264 was isolated from the oyster mushroom damaged by the disease in Korean. In order to isolate tolaasin molecules, the supernatant of bacterial culture was harvested at the stationary phase of growth. Tolaasin was prepared by ammonium sulfate pre...

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A toxin produced by Pseudomonas tolaasii, tolaasin, causes brown blotch disease in mushrooms. Tolaasin forms pores on the cellular membrane and destroys cell structure. Inhibiting the ability of tolaasin to form ion channels may be an effective method to protect against attack by tolaasin. However, it is first necessary to elucidate the three-dimensional structure of the ion channels formed by ...

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Introduction: Chemotherapy has been restricted due to the high-dose side effects. In the present study, acceleration of the chemotherapeutic drug (5FU) entrance into MCF-7 cells has been explored by using a recombinant form of Fragaceatoxin C (FraC) pore-forming toxin. Methods: In this experimental study, the gene for FraC toxin was order from a commercial source and was sub-cloned into pET28a...

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ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2020

ISSN: 0006-3495

DOI: 10.1016/j.bpj.2019.11.2182